Journal: Nature Communications
Article Title: Electron-donable heterojunctions with synergetic Ru-Cu pair sites for biocatalytic microenvironment modulations in inflammatory mandible defects
doi: 10.1038/s41467-024-53824-y
Figure Lengend Snippet: Fluorescence staining images of ( a ) TNF-α and ( b ) BMP2/4 at week 1 after treatments. Scale bar: 200 μm. c Schematic illustration of Ru-Cu/EDHJ for ROS scavenging and promoting bone formation. Quantitative results of fluorescence intensity of ( d ) TNF-α ( n = 3 independent replicates), p (ROSup) = 0.0499, compared with Ctrl group; p (Ru-Cu/EDHJ-ROSup ) = 0.0175, compared with ROSup group. Quantitative results of fluorescence intensity of ( e ) BMP2/4 ( n = 3 independent replicates), p (ROSup) = 0.0202, compared with Ctrl group; p (Ru-Cu/EDHJ-ROSup) = 0.0004, compared with ROSup group. f 3D reconstruction of micro-CT images. Scale bar: 1 mm. New bone formation quantitative result of ( g ) BV/TV ( n = 3 independent replicates), p (ROSup, 2w) = 0.0013, p (ROSup, 4w) = 0.0348, p (ROSup, 8w) = 0.0013, compared with Ctrl group; p (Ru-Cu/EDHJ-ROSup, 2w) < 0.0001, p (Ru-Cu/EDHJ-ROSup, 4w) < 0.0001, p (Ru-Cu/EDHJ-ROSup, 8w) < 0.0001, compared with ROSup group. New bone formation quantitative result of ( h ) bone mineral density (BMD) ( n = 3 independent replicates), p (ROSup, 2w) = 0.0295, p (ROSup, 4w) = 0.0018, p (ROSup, 8w) = 0.0461, compared with Ctrl group; p (Ru-Cu/EDHJ-ROSup, 2w) = 0.0112, p (Ru-Cu/EDHJ-ROSup, 4w) < 0.0001, p (Ru-Cu/EDHJ-ROSup, 8w) = 0.0003, compared with ROSup group. New bone formation quantitative result of ( i ) Tb.N ( n = 3 independent replicates), p (ROSup, 2w) = 0.0003, p (ROSup, 4w) < 0.0001, p (ROSup,8w) = 0.0435, compared with Ctrl group; p (Ru-Cu/EDHJ-ROSup, 2w) < 0.0001, p (Ru-Cu/EDHJ-ROSup, 4w) < 0.0001, p (Ru-Cu/EDHJ-ROSup, 8w) = 0.0115, compared with ROSup group. New bone formation quantitative result of ( j ) Tb.Th ( n = 3 independent replicates), p (ROSup,2w) = 0.0206, p (ROSup,4w) = 0.0401, p (ROSup, 8w) < 0.0001, compared with Ctrl group; p (Ru-Cu/EDHJ-ROSup, 2w) < 0.0001, p (Ru-Cu/EDHJ-ROSup, 4w) = 0.0096, p (Ru-Cu/EDHJ-ROSup, 8w) < 0.0001, compared with ROSup group. Ctrl (mandible defects with PBS treatment). Data are presented as means ± SD., * p < 0.05, ** p < 0.01, *** p < 0.001, # p < 0.05, ## p < 0.01, ### p < 0.001, ns, no significance; statistical significance was calculated using one-way ANOVA followed by Tukey’s post-hoc test for multiple com p arisons, all tests were two-sided. Experiments were repeated independently ( a, b, f ) three times with similar results. Source data are provided as a Source Data file.
Article Snippet: Primary antibodies and corresponding concentrations used in this study were 4-HNE (MA5-27570, Life Technologies, USA,1:100 dilution), paxillin (ab32084, Abcam, USA, 1:200 dilution), γH2A.X (phospho S139) (ab81299, Abcam, USA, 1:200 dilution), DNA/RNA Damage (ab62623, Abcam, USA, 1:200 dilution), and COL1A1 (R26615, ZEN-BIOSCIENCE, China, 1:50 dilution), TNFα (346654, ZEN-BIOSCIENCE, China, 1:50 dilution), BMP2/4 (sc-137087, Santa Cruz Biotechnology, USA, 1:50 dilution), CD140a (ab203491, Abcam, USA, 1:200 dilution), iNOS (GB11119, Servicebio, China, 1:50), CD206 (sc-58986, Santa Cruz Biotechnology, USA, 1:50 dilution), Ly6g (GB11229, Servicebio, China, 1:50).
Techniques: Fluorescence, Staining, Micro-CT
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